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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: Breast cancer-derived CAV1 promotes lung metastasis by regulating integrin α6β4 and the recruitment and polarization of tumor-associated neutrophils
doi: 10.7150/ijbs.94153
Figure Lengend Snippet: CAV1 Promotes BC Lung Metastasis by Activating the Src/FAK/α6β4 Pathway in MDA-MB-231 Cells and Simultaneously Activating Src/PI3K Signaling Downstream of α6β4 in Lung Epithelial Cells. a: CCK8 assay was used to detect the optimal acting concentration of the Src inhibitor PP2. b-e: Western blot analysis was performed to detect the expression of CAV1 and integrin Src/FAK/α6β4 signaling pathway after overexpression, knockdown, and addition of PP2. f: Western blot was used to detect the activation of integrin α6β4 downstream signaling in lung epithelial cells. g,h: IHC staining was used to detect the activation of integrin α6β4 downstream signaling PI3K/Src in mice lungs. Bar=100um. Data ware shown as mean ± SD and assessed with One-way ANOVA test. (n=3) (ns stands for non-significant difference; *p<0.05; **p<0.01; ***p<0.001).
Article Snippet: Caveolin1 Y14 phosphorylation was inhibited by the
Techniques: CCK-8 Assay, Concentration Assay, Western Blot, Expressing, Over Expression, Knockdown, Activation Assay, Immunohistochemistry